ACS Biochemistry Practice Exam 2026 - Free Biochemistry Exam Practice Questions and Study Guide

The main purpose of using FMOC (9-fluorenylmethoxycarbonyl) in chemical synthesis is to protect the N-terminus during the synthesis of amino acids. In the context of peptide synthesis, it serves as a protecting group that prevents unwanted reactions at the N-terminus, allowing for selective reactions to occur at the C-terminus or side chains of the amino acids.

FMOC is particularly advantageous due to its stability and ease of removal; it can be removed using mild conditions, such as a base, which is suitable for protecting groups in peptide synthesis. This characteristic makes it a popular choice among chemists when constructing peptides and proteins stepwise while ensuring that the N-terminus remains unreacted until the appropriate stage of synthesis.

Understanding the role of FMOC in protecting the N-terminus is crucial for anyone studying biochemical synthesis, as it highlights the importance of protecting groups in obtaining the desired products without undesired side reactions.